Introduction Plasmids Bacteriophage Lambda Vectors Cosmids Yeast Artificial Chromosomes (YACs) Bacterial Artificial Chromosomes (BACs) Library Screening and Gene Sequencing Course Topics Course Home Page

Introduction What is cloning? Cloning is a procedure which generates a large number of copies of a single sequence of DNA. These procedures depend upon the ability of vectors to continue their life cycles in bacterial or yeast cells in spite of having foreign DNA inserted into them. Because the foreign DNA is carried into the bacterial or yeast cell by these molecules, they are called cloning vectors. Properties of all cloning vectors will accept foreign DNA and can still complete their life cycle permit those bacterial or yeast cells that contain the foreign DNA to be distinguished from those that do not Types of vectors plasmids bacteriophage cosmids yeast artificial chromosomes (YACs) bacterial artificial chromosomes (BACs) Insert Size of Various DNA Cloning Vectors Vector Insert size (kb) Plasmid <10 kb Bacteriophage 9-20 kb Cosmids 33-47 kb BACs 75-125 kb YACS 100-1000 kb Copyright © 1998. Phillip McClean