Buddhadev Layek, PhD
Assistant Professor
116B Sudro Hall
701.231.7906
Education
2009-2014, PhD (Pharmaceutical Sciences), North Dakota State University, Fargo, ND
2004-2006, Master of Pharmacy (Pharmaceutics), Jadavpur University, Kolkata, India
2000-2004, Bachelor of Pharmacy, Jadavpur University, Kolkata, India
Teaching
PSCI 367, Pharmaceutical Calculations
PSCI 369, Pharmaceutics II
PSCI 411/611, Principles of PK/PD
Research Interests
- Design, development, and characterization of polymeric nanocarrier-based formulations for targeted delivery of small molecule drugs, plasmid DNA, siRNA, proteins, and peptides for the treatment of cancer and neurological disorders.
- Stem cell-based therapies for regenerative medicine, immunotherapy, and tumor-targeted delivery of chemotherapeutics.
- Pharmacokinetic, toxicokinetic, and metabolic profiling of small molecule drug candidates and biologics.
Academic and Professional Appointments
2/2020-present, Assistant Professor, Department of Pharmaceutical Sciences, School of Pharmacy, North Dakota State University, Fargo, ND
12/2017-01/2020, Researcher 5, College of Pharmacy, University of Minnesota, Minneapolis, MN
5/2014-12/2017, Postdoctoral Associate, College of Pharmacy, University of Minnesota, Minneapolis, MN
7/2006-12/2008, Junior Scientist, Dr. Reddy's Laboratories Ltd, Hyderabad, India
Professional Memberships
American Association for Cancer Research, 2017-Present
American Association of Pharmaceutical Scientists (AAPS), 2009-Present
Awards/Honors
2018, Best Poster Presentation Award, 50th Pharmaceutics Graduate Research Meeting, Minneapolis, MN
200, 2017 American Association of Pharmaceutical Scientists (AAPS) Travel Award
2016, Baxter Young Investigator Award, Baxter Healthcare, IL
2014, The Graduate School Research Award, North Dakota State University, Fargo, ND
2012-2014, Doctoral Dissertation Assistantships Award, North Dakota Experimental Program to Stimulate Competitive Research (ND EPSCoR)
2004-2006, Junior Research Fellowship, University Grants Commission, India
2004, Prof. Anupam Sengupta Memorial Medal, Jadavpur University, India
Patent
Prabha S, Sadhukha T, and Layek B. Targeted delivery system, system components, and methods. US Patent App. 15/434,640.
Peer-reviewed Publications
1. Layek B, Mandal S. Natural polysaccharides for controlled delivery of oral therapeutics: a recent update. Carbohydr. Polym. 2020, 230: 115617.
2. Layek B, Sehgal D, Panyam J, Prabha S. Nanoengineering of mesenchymal stem cells via surface modification for efficient cancer therapy. Advanced Therapeutics 2019, 2, 1900043.
3. Elie BT, Hubbard K, Pechenyy Y, Layek B, Prabha S, Contel M. Preclinical evaluation of an unconventional ruthenium-gold-based chemotherapeutic: RANCE-1, in clear cell renal cell carcinoma. Cancer Med. 2019, 8(9), 4304-4314.
4. Cheng S, Nethi SK, Rathi S, Layek B, Prabha S. Engineered mesenchymal stem cells (MSCs) for targeting solid tumors: therapeutic potential beyond regenerative therapy. J. Pharmacol. Exp. Ther., 2019, 370(2), 231-241.
5. Moku G, Layek B, Trautman L, Putnam S, Panyam J, Prabha S. Improving payload capacity and anti-tumor efficacy of mesenchymal stem cells using TAT peptide functionalized polymeric nanoparticles. Cancers 2019, 11(4), 491. (co‐first author)
6. Layek B, Rahman Nirzhor SS, Rathi S, Wiedmann TS, Kandimalla K, Prabha S. Design, development, and characterization of imiquimod-loaded chitosan films for topical delivery. AAPS PharmSciTech, 2019, 20 (2), 58.
7. Layek B, Sadhukha T, Panyam J, Prabha S. Nano-engineered mesenchymal stem cells increase therapeutic efficacy of anticancer drug through true active tumor targeting. Mol. Cancer Ther. 2018, 17(6), 1196-1206.
8. Sadhukha T, Layek B, Prabha S. Incorporation of lipolysis in monolayer permeability studies of lipid-based oral drug delivery systems. Drug Deliv. Transl. Res. 2018, 8(2), 375-386. (co‐first author)
9. Layek B, Sadhukha T, Prabha S. Glycoengineered mesenchymal stem cells as an enabling platform for two-step targeting of solid tumors. Biomaterials 2016, 88, 97-109.
10. Usacheva M, Layek B, Rahman Nirzhor SS, Prabha S. Nanoparticle-mediated photodynamic therapy for mixed biofilms. J. Nanomater. 2016, 2016, 11.
11. Layek B, Lipp L, Singh J. Cell penetrating peptide conjugated chitosan for enhanced delivery of nucleic acid. Int. J. Mol. Sci. 2015, 16(12), 28912-28930.
12. Layek B, Lipp L, Singh J. APC targeted micelle for enhanced intradermal delivery of hepatitis B DNA vaccine. J. Control. Release 2015, 207, 143-153.
13. Nahire R, Haldar MK, Paul S, Ambre AH, Meghnani V, Layek B, Katti KS, Gange KN, Singh J, Sarkar K. Multifunctional polymersomes for cytosolic delivery of gemcitabine and doxorubicin to cancer cells. Biomaterials 2014, 35(24), 6482-6497.
14. Layek B, Haldar MK, Sharma G, Lipp L, Mallik S, Singh J. Hexanoic acid and polyethylene glycol double grafted amphiphilic chitosan for enhanced gene delivery: Influence of hydrophobic and hydrophilic substitution degree. Mol. Pharm. 2014, 11(3), 982-994.
15. Layek B, Singh J. Cell penetrating peptide conjugated polymeric micelles as a high performance versatile nonviral gene carrier. Biomacromolecules 2013, 14(11), 4071-4081.
16. Layek B, Singh, J. Caproic acid grafted chitosan cationic nanocomplexes for enhanced gene delivery: Effect of degree of substitution. Int. J. Pharm. 2013, 447(1-2), 182-191.
17. Sharma G, Modgil A, Layek B, Arora K, Sun C, Law B, Singh J. Cell penetrating peptide tethered bi-ligand liposomes for delivery to brain in vivo: Biodistribution and transfection. J. Control. Release 2013, 167, 1-10.
18. Layek B, Singh J. Amino acid grafted chitosan for high performance gene delivery: Comparison of amino acid hydrophobicity on vector and polyplex characteristics. Biomacromolecules 2013, 14, 485-494.
19. Layek B, Singh J. N-hexanoyl, n-octanoyl and n-decanoyl chitosans: Binding affinity, cell uptake, and transfection. Carbohydr. Polym. 2012, 89, 403-410.
20. Layek B, Mukherjee B. Tamoxifen citrate encapsulated sustained release liposomes: Preparation and evaluation of physicochemical properties. Sci. Pharm. 2010, 78, 507-515.
21. Vittal S, Ganneboina R, Layek B, Trivedi RK, Hotha KK, Bharathi DV, Mullangi R. Highly sensitive method for the determination of omeprazole in human plasma by liquid chromatography–electrospray ionization tandem mass spectrometry: Application to a clinical pharmacokinetic study. Biomed. Chromatogr. 2009, 23(4), 390-396.
22. Layek B, Kumar TS, Trivedi RK, Mullangi R, Srinivas NR. Development and validation of a sensitive LC‐MS/MS method with electrospray ionization for quantitation of rhein in human plasma: Application to a pharmacokinetic study. Biomed. Chromatogr. 2008, 22(6), 616-624.
23. Trivedi RK, Layek B, Kumar TS, Vittal S, Ganneboina R, Dubey P, Mullangi R, Srinivas NR. Chiral bioanalysis of torcetrapib enantiomers in hamster plasma by normal-phase liquid chromatography and detection by atmospheric pressure chemical ionization tandem mass spectrometry. J. Chromatogr. B 2007, 860, 227-234.
24. Mukherjee B, Patra B, Layek B, Mukherjee A. Sustained release of acyclovir from nano-liposomes and nano-niosomes: An in vitro study. Int. J. Nanomed. 2007, 2(2), 213-225.
25. Mukherjee B, Das S, Patra B, Layek B. Nefopam containing transdermal-matrix patches based on pressure- sensitive adhesive polymer. Pharm. Tech. 2006, 30(3), 146-163.
Editorial
1. Layek B, Singh J. Editorial of Special Issue "Surface-Functionalized Nanoparticles as Drug Carriers". Int. J. Mol. Sci. 2019;20(24):6352.
Book Chapters
1. Babu M, Layek B, Singh J. Electroporation for dermal and transdermal drug delivery. In Percutaneous penetration enhancers physical methods in penetration enhancement, Dragicevic N and Maibach HI, Eds., Springer, 2017, 105-122.
2. Layek B, Singh J. Chitosan for DNA and gene therapy. In Chitosan based biomaterials volume 2, Jennings JA and Bumgardner JD, Eds., Elsevier, 2016, 209-244.
3. Layek B, Paul S, Singh J. Prodrug strategy: an effective tool in drug delivery and targeting. In Bio-targets and drug delivery approaches, Maiti S and Sen KK, Eds., CRC Press (Taylor & Francis Group), 2016, 77-111.
4. Oak M, Mandke R, Layek B, Sharma G, Singh J. Controlled‐release systems for biologics. In Biological drug products: Development and strategies, Wang W and Singh M, Eds., John Wiley Publishers, 2013, 655-676.
5. Tang Y, Oak M, Mandke R, Layek B, Sharma G, Singh J. Thermosensitive polymers for controlled delivery of hormones. In Active implants and scaffolds for tissue regeneration, Zilberman M Ed., Springer, 2011, 457-479.
6. Mandke R, Layek B, Sharma G, Singh J. Fabrication and evaluation of nanoparticles-based biosensors. In Biosensor nanomaterials, Li S et al., Eds., Wiley Publishers, 2011, 73-93.
Conference Presentations
1. Cheng S, Layek B, Al-Kofahi M, Brundage R, Prabha S. Elucidating the disposition of nano-engineered mesenchymal stem cells using pharmacokinetic modeling. American Conference on Pharmacometrics, Orlando, FL, USA, 2019.
2. Layek B, Sehgal D, Panyam J, Prabha S. Glycoengineered MSCs for targeting platinum resistant ovarian tumors. American Association for Cancer Research (AACR) Annual Meeting, Atlanta, GA, USA, 2019.
3. Prabha S, Moku G, Layek B, Panyam J. Mesenchymal stem cells engineered with TAT peptide functionalized nanoparticles improve therapeutic efficacy of paclitaxel in an orthotopic lung tumor model. AACR Annual Meeting, Atlanta, GA, USA, 2019.
4. Cheng S, Layek B, Al-Kofahi M, Brundage R, Prabha S. Elucidating the disposition of nano-engineered mesenchymal stem cells using pharmacokinetic modeling. MCC Research Symposium, Minneapolis, MN, USA, 2019.
5. Layek B, Rahman Nirzhor SS, Wiedmann T, Prabha S. Design, development, and characterization of imiquimod-loaded chitosan films. PharmSci 360, Washington, D.C., USA, 2018.
6. Layek B, Shetty M, Starr TK, Prabha S. A two-step targeting strategy utilizing glycoengineered mesenchymal stem cells for ovarian cancer. 12th Biennial Ovarian Cancer Research Symposium, Seattle, WA, USA, 2018.
7. Layek B, Panyam J, Prabha S. Nanoengineered mesenchymal stem cells exhibit enhanced anti-tumor efficacy in a syngeneic mouse model. 50th Pharmaceutics Graduate Research Meeting, Minneapolis, MN, USA, 2018.
8. Layek B, Shetty M, Starr TK, Prabha S. Translational studies on glycoengineered mesenchymal stem cells in ovarian cancer. MCC Research Symposium, Minneapolis, MN, USA, 2018.
9. Layek B, Panyam J, Prabha S. Nanoengineered mesenchymal stem cells successfully inhibit tumor progression in a syngeneic mouse model. AACR Annual Meeting, Chicago, IL, USA, 2018.
10. Layek B, Meena J, Prabha S. Glycoengineered mesenchymal stem cells potentiates antitumor efficacy of paclitaxel. AAPS Annual Meeting, San Diego, CA, USA, 2017.
11. Prabha S, Sadhukha T, Layek B. Nanoengineered mesenchymal stem cells for targeted lung cancer therapy. AACR Annual Meeting, Washington, D.C., USA, 2017.
12. Layek B, Sadhukha T, Prabha S. Glycoengineered mesenchymal stem cells as an enabling platform for two-step targeting of solid tumors. GPEN, Lawrence, Kansas, USA, 2016 (podium).
13. Layek B, Sadhukha T, Prabha S. Glycoengineered MSCs as an enabling platform for two-step targeting of solid tumors. 48th Pharmaceutics Graduate Student Research Meeting (PGSRM), Kansas City, MO, USA, 2016 (podium).
14. Layek B, Prabha S. Glycoengineered mesenchymal stem cells as an enabling platform for two-step targeting of solid tumors. MCC Research Symposium, Minneapolis, MN, USA, 2015.
15. Layek B, Sadhukha T, O’Brien TD, Prabha S. Glycoengineered MSCs as potential theranostic agents: process optimization. AAPS Annual Meeting, Orlando, FL, USA, 2015.
16. Sadhukha T, Layek B, O'Brien TD, Prabha S. Glyco-engineered mesenchymal stem cells as potential theranostic agents: therapeutic nanoparticle conjugation. Nano Engineering for Medicine and Biology Conference, Minneapolis, MN, USA, 2015.
17. Layek B, Sadhukha T, O'Brien TD, Prabha S. Glycoengineered MSCs as potential theranostic agents: process optimization. Nano Engineering for Medicine and Biology Conference, Minneapolis, MN, USA, 2015.
18. Layek B, Singh J. Hexanoic acid grafted mannosylated chitosan for targeted DNA vaccine delivery to dendritic cells. AAPS Annual Meeting, San Diego, CA, USA, 2014.
19. Layek B, Lipp L, Singh J. Antigen presenting cells targeted cationic micelle for enhanced intradermal delivery of hepatitis B DNA vaccine. AAPS Annual Meeting, San Diego, CA, USA, 2014.
20. Lipp L, Layek B, Lakkadwala S, Singh J. Synthesis and characterization of triblock copolymers and their use in controlled release of incorporated salmon calcitonin, in vitro. AAPS Annual Meeting, San Diego, CA, USA, 2014.
21. Layek B, Singh J. Cell penetrating peptide conjugated polymeric nanomicelles as high performance nonviral gene delivery system. AAPS Annual Meeting, San Antonio, TX, USA, 2013.
22. Layek B, Lipp L, Singh J. Hexanoyl chloride and polyethylene glycol modified chitosan for enhanced gene delivery: influence of degree of substitutions. AAPS Annual Meeting, San Antonio, TX, USA, 2013.
23. Layek B, Lipp L, Singh J. Targeted gene and DNA vaccine delivery to macrophage. AAPS Annual Meeting, San Antonio, TX, USA, 2013.
24. Layek B, Singh J. Effective DNA vaccine delivery to antigen presenting cells via mannose receptor targeted phenylalanine grafted chitosan. AAPS Annual Meeting, San Antonio, TX, USA, 2013.
25. Lipp L, Layek B, Singh J. Synthesis, characterization, and biocompatibility of triblock copolymers. AAPS Annual Meeting and Exposition, San Antonio, TX, USA, 2013.
26. Lipp L, Layek B, Singh J. Synthesis, characterization, and biocompatibility of triblock copolymers. 45th PGSRM, Iowa City, IA, USA, 2013.
27. Layek B, Singh J. Synergistic effect of conjugating cell penetrating peptide and linoleic acid on gene transfection efficiency of chitosan. Graduate Research and Arts Forum, North Dakota State University, ND, USA, 2013.
28. Layek B, Singh J. N-hexanoyl chitosan for enhanced gene delivery: effect of degree of substitution. AAPS Annual Meeting and Exposition, Chicago, IL, USA, 2012.
29. Layek B, Singh, J. Synthesis and characterization of phenylalanine grafted chitosan for gene delivery. AAPS Annual Meeting, Chicago, IL, USA, 2012.
30. Layek B, Singh J. Effect of amino acid alkyl chain length on cellular uptake and transfection efficiency of hydrophobically modified chitosan. AAPS Annual Meeting and Exposition, Chicago, IL, USA, 2012.
31. Layek B, Singh J. Mannosylated phenylalanine-graft-chitosan for targeted gene delivery to antigen presenting cells. ND EPSCoR State Conference, Grand Forks, ND, USA, 2012.
32. Layek B, Singh J. N-hexanoic acid grafted cationic nanomicelles for enhanced gene transfection: effect of degree of substitution. 44th PGSRM, Omaha, NE, USA, 2012.
33. Layek B, Singh J. Effect of fatty acid chain length on transfection efficiency of hydrophobically modified low molecular weight chitosan. AAPS Annual Meeting, Washington, D.C., USA, 2011.
34. Layek B, Singh J. Caprylic acid grafted low molecular weight chitosan for improved gene transfection: effect of degree of substitution. AAPS Annual Meeting, Washington, D.C., USA, 2011.
35. Layek B, Singh J. Evaluation of hydrophobically modified low molecular weight chitosan as a potential non-viral vector for gene delivery. 43rd PGSRM, Madison, WI, USA, 2011.
36. Layek B, Singh J. Cationic nanomicelles to deliver DNA vaccine. AAPS Annual Meeting, New Orleans, LA, USA, 2010.
37. Oak M, Layek B, Singh J. Synthesis and characterization of triblock copolymers for the controlled delivery of insulin. AAPS Annual Meeting, Los Angeles, CA, USA, 2009.
38. Oak M, Layek B, Singh J. Synthesis and characterization of triblock copolymers for the controlled delivery of insulin. 41st PGSRM, Purdue, IN, USA, 2009.
Professional Services
Editorial Activities:
- Guest editor of International Journal of Molecular Sciences (Cell-penetrating peptides 2015 and 2016 and Surface-functionalized nanoparticles as drug carriers, 2019)
- Co-editing a book entitled “Tailor-made and functionalized biopolymer systems for drug delivery and biomedical applications.” Elsevier (under process)
Ad hoc Reviewer:
ACS Omega, Acta Biomaterialia, Biomacromolecules, Biomolecules, Colloids and Surfaces B: Biointerfaces, Carbohydrate Polymers, Current Cancer Drug Targets, International Journal of Molecular Sciences, International Journal of Nanomedicine, International Journal of Pharmaceutics, International Journal of Biological Macromolecules, Journal of Chromatography B, Journal of Drug Delivery Science and Technology, Marine Drugs, Materials Science and Engineering: C, Molecular Pharmaceutics, Nanomaterials, Pharmaceutics.