Current Topics in Genomics - Spring, 2022

Date: Wednesday, May 4, 2022
Time: 1:30 pm
Mode: Zoom (link will be provided later)

Topic: Mapping the Metabolome in Plants

Plants produce a wide-array of metabolites. As with any molecule, their production are essential in some manner to the life cycle of the invidual plant. As with phenotype, the production of these metabolites, they are under the control of some genetic factor in the genome. This session of Current Topics in Genomics will focus on the techniques to measure the metabolom and genetic approach to map the genetic factor for a metabolite in the genome. One paper will describe the general experimental approaches to isolating single cell types and their analysis. Another paper will discuss the application of these techniques to lateral root development in Arabidopsis. The third paper will describe the development of a cis-regulatory atlas across six different maize organs from data collected at the single-cell level.

Presentation Instructions

Each participant needs to present a description of the paper that lasts 20-25 minutes. This presentation is required to be in the form of a MS Powerpoint presentation. The presentation should 1) provide an introduction to the methids used to phenotype the metabolome; 2) outline the experimental methodology, and 3) include a discussion of the key tables and figures in the paper and how they relate to the theme of the paper.

Each student will also be required to lead the discussion of the paper after the presentation. As discussion leader, you must also be familiar with the content of the second paper. All assignments are below

Papers

Kennedy Lund: Alseekh and Fernie - Metabolomics 20 years on: What have we learned and what hurdles remain (Discussion leader: Josephine Princy Johnson)

Josephine Princy Johnson: Chen et al - Comparative and parallel genome-wide association studies for metabolic and agronomic traits in cereals (Discussion leader: Mario Morales)

Mario Morales: Wu et al - Mapping the Arabidopsis Metabolic Landscape by Untargeted Metabolomics at Different Environmental Conditions (Discussion leader: Kennedy Lund)