Next Generation Sequencing
NextSeq1000
The Illumina NextSeq1000 enables flexible choices to handle many of your Next Generation Sequencing (NGS) needs. We have used these machines to sequence microbial metagenomes, transcriptomes and 16S taxonomic profiles from a variety of sources (soil, plants, animals, water, cultures). While we specialize in microbe sequencing in our Department, these instruments can be used for a wide array of sequencing projects. The Biotech Innovation Core is equipped with all of the ancillary equipment required to create, quantify and perform QA/QC on NGS libraries.
Sequencing output per flow cell with XLEAP-SBS reagents
Flow Cell Type | P1 | P2 |
|---|---|---|
2x50bp | 10 Gb | 40 Gb |
2x100bp | n/a | 80 Gb |
2x150bp | 30 Gb | 120 Gb |
2x300bp | 60 Gb | 240 Gb |
Estimated Sample throughput
Application | P1 # of samples | P2 # of samples |
|---|---|---|
130 Mb genome, 30x coverage | 7 | 30 |
mRNA seq, 25M reads per sample (200 cycle) | 4 | 16 |
16S or ITS | 384a | 384a |
a limited by the number of unique dual indexes available commercially, can add many more if using your own indexes
Tapestation 4150
The TapeStation Bioanalyzer allows for precisely sizing and quantifying DNA and RNA samples. Users are required to bring their own reagents.
