An “HG type,’” named after the genus and species name (Heterodera glycines), was developed to identify nematode populations that begin to adapt to genetic resistance. Importantly, the HG type alone does not tell us how much the SCN population has adapted to a resistance source, only that the process has begun. “Races” were (and in some cases still are) used to classify differences in SCN populations before the HG type was developed. HG type and races are similar, but the HG type test incorporates the seven breeding lines that are available as sources of resistance and makes room for more as they are developed. For information on converting a race name into an HG type, please visit www.thescncoalition.com.
The HG type tests characterize the ability of SCN populations to reproduce on seven different soybean breeding lines known to have resistance to SCN. These are called “indicator lines,” and each has a numeric designation (1 to 7). HG type tests are conducted under greenhouse conditions by growing each of the seven indicator lines in soil infested with a SCN sample population. A female index (FI), which is the percentage of reproduction on each line, relative to the susceptible check, is calculated on each indicator line.
If the female index is 10% or greater, than an indicator line is considered susceptible. Let’s consider three examples:
Example 1. A SCN population was tested, and FI values were less than 10% on all indicator lines tested. This population would be designated as HG type 0. In this example (HG type 0), SCN population has not overcome the resistance provided by any of the seven indicator lines. Currently, a majority of the SCN populations tested from North Dakota were HG type 0.
Example 2. An HG type test reveals that a SCN population had FI values greater than 10% on indicator lines 2, 5 and 7, while having less than 10% FI values on all other indicator lines. This population would be designated as HG type 2.5.7. This demonstrates that an increase in SCN reproduction is beginning to occur on soybean varieties that derive resistance from indicator lines number 2 (PI 88788), 5 (PI 209332) and 7 (PI 548316). Currently, several populations in North Dakota were discovered with increased reproduction on PI 88788 (indicator line 2), the most commonly used source resistance in commercial soybean varieties. Elevated reproduction has also been observed on indicator lines 5 and 7, but because those sources of resistance are not used in commercial soybean varieties, increased reproduction is not currently a concern.
Example 3. An HG type test reveals FI values greater than 10% on indicator lines 1 and 2 but values less than 10% on the remaining five lines. This population would be designated HG type 1.2. The implication of this HG type is that the nematode is beginning to overcome resistance on the two most important sources of resistance to SCN, PI548402 (Peking) (indicator line 1) and PI88788 (indicator line 2). Recent surveys found several SCN populations from North Dakota reproducing on PI88788, beginning to overcome the PI88788-type resistance, but very few were able to overcome Peking-type resistance.
For practical purposes, the most important numbers to pay attention to in an HG type are 1 and 2. When 1 is present, the nematode population is beginning to overcome the resistance in ‘Peking’. When a 2 is present, the nematode is beginning to overcome the resistance in ‘PI88788’.