lean, sanitize and disinfect reusable PPE and equipment. Different cleaning processes vary in their degree of pathogen destruction. The images in Figure 3 compare the soles of footwear and the effectiveness of three cleaning methods. Choose the most effective method and product. Figure 4 describes the level of effectiveness from cleaning to sterilizing.
Figure 3. Demonstration of bacteria cultured from boots with smooth and patterned soles. 1 = dirty, 2 = washed with water, 3 = disinfected. Note that patterned-soled boots are more difficult to disinfect effectively and may still harbor bacteria.
CLEANING
Cleaning involves the physical removal of visible contamination from surfaces. Soaps and detergents bind to oils, so
Figure 4. Levels of destruction. Adapted from “Disinfection 101” from The Center for Food Security and Public Health, Iowa State University College of Veterinary Medicine.
SANITIZING
Sanitizing significantly reduces bacterial contamination on surfaces to levels considered safe from a public health standpoint. It does not eliminate all microorganisms. Sanitizers are most commonly used for food contact surfaces.
DISINFECTION
Disinfection destroys or irreversibly inactivates most pathogens (e.g., bacteria, viruses and fungi) on surfaces (i.e., inanimate objects). It is generally not effective against bacterial spores. Efficacy will vary with disinfectant product or method.
STERILIZATION
Sterilization destroys or eliminates all forms of microbial life, including bacterial spores. This involves the use of higher levels of physical (e.g., extreme heat) or chemical (liquid or gas sterilants) processes and is generally used for medical devices/equipment.
© CFSPH 2008-2023
For effective sanitizing and disinfecting results, clean all surfaces first.
Between site visits, clean then disinfect reusable equipment using a guideline in Figure 5. Make these solutions daily with fresh water. You can buy premixed products with the active ingredient sodium hypochlorite (bleach) or hydrogen peroxide. Always read the manufacturers label for proper use.
This information is based on site visits where there is no known pathogen risk. An outbreak would require more rigorous cleaning and disinfecting. For disinfecting information for various pathogens, visit “Select Animal Diseases Disinfectant Chart” (NDSU V2128) or the USDA APHIS website for comprehensive information.
Figure 5. Simple bleach and hydrogen peroxide dilutions. Adapted from “Disinfection 101: Key Principles of Cleaning and Disinfection for Animal Settings.” The Center for Food Security and Public Health, Iowa State University College of Veterinary Medicine, 2023
Select Animal Disease Disinfectant Chart