Page Title

Sequencers

Description

NextSeq1000

scientist starting sequencing run on NextSeq 1k/2k

The Illumina NextSeq1000 enables flexible choices to handle many of your Next Generation Sequencing (NGS) needs. We have used these machines to sequence microbial metagenomes, transcriptomes and 16S taxonomic profiles from a variety of sources (soil, plants, animals, water, cultures). While we specialize in microbe sequencing in our Department, these instruments can be used for a wide array of sequencing projects. The Biotech Innovation Core is equipped with all of the ancillary equipment required to create, quantify and perform QA/QC on NGS libraries.

Sequencing output per flow cell with XLEAP-SBS reagents

Flow Cell Type

P1

P2

2x50bp

10 Gb

40 Gb

2x100bp

n/a

80 Gb

2x150bp

30 Gb

120 Gb

2x300bp

60 Gb

240 Gb

Estimated Sample throughput

Application

P1 

# of samples

P2

# of samples

130 Mb genome, 30x coverage

7

30

mRNA seq, 25M reads per sample (200 cycle)

4

16

16S or ITS

384a

384a

limited by the number of unique dual indexes available commercially, can add many more if using your own indexes

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We strive to make advanced biotechnology tools accessible to all researchers. For any questions or additional information, please get in touch with us at NDSU.biotechcore@ndsu.edu.